Additional file 1: of Combined treatment with HMGN1 and anti-CD4 depleting antibody reverses T cell exhaustion and exerts robust anti-tumor effects in mice

Abstract

Figure S1. The purification of recombinant HMGN1 proteins. Figure S2. Administration of human HMGN1 (hH) with anti-CD4 depleting antibody (αCD4) exerted anti-tumor effects in mice. Figure S3. Administration of murine HMGN1 with anti-CD4 antibody exerted anti-tumor effects in melanoma model. (A). Figure S4. Flow cytometry analysis of T cell populations in the draining lymph node (dLN) from Colon26 tumor-bearing mice on day 13 after tumor inoculation. Figure S5. Gating scheme of CCR7+ CD11b+ migratory dendritic cells (DCs). Figure S6. Flow cytometry analysis of exhausted CD8+ T cell populations in the tumor from tumor-bearing mice on day 17 after treatment. Figure S7. CD8 T cell transcriptome analysis revealed the promotion of activation after HMGN1/αCD4 treatment. Method S1. Production and purification of HMGN1 in E. coli. Method S2. 3’end Serial Analysis of Gene Expression (SAGE) sequencing library preparation. Table S1. The list of recombinant proteins and peptides. Table S2. Antibodies for flow cytometry. Table S3. Primers for quantitative real-time PCR. Table S4. Gene ontology (GO) analysis of GO: biological processes and KEGG pathway. (PDF 2580 kb