Interaction between TYLCV C2 and AtRPS27A affects whitefly performance.

Abstract

(A) In vitro GST pull-down assays. MBP or MBP- C2 fusion proteins were pull-down by GST or GST-AtRPS27A fusion protein. GST beads were washed and proteins were analyzed by SDS-PAGE western blot. Associated proteins were detected by anti-MBP antibody and gels were stained with Coomassie Brilliant Blue to monitor GST and GST fusion proteins. (B) In vivo BiFC analysis of C2 interaction with NtRPS27A. Nuclei of tobacco leaf epidermal cells were marked with a RFP fusion protein. Bars = 20 mm. (C) Subcellular localization of C2 and AtRPS27A. Nuclei of tobacco leaf epidermal cells were marked with a RFP fusion protein. Bars = 20 mm. (D) Survival rate of adult whitefly on wild type Arabidopsis plants and the transgenic C2 expressing Arabidopsis plants. Values are means± SE, n = 30. (E) Daily number of eggs laid by per female whitefly on wild type Arabidopsis plants and the transgenic Arabidopsis plants expressing C2. Values are means±SE, n = 30. Asterisks indicate significant differences between different treatments (P < 0.05; Student’s t test for all experiments). All experiments were repeated three times with similar results.</p