Effect of fluid velocity on bacterial binding to mucins.

Abstract

A and B. A. salmonicida binding to Atlantic salmon skin and distal intestinal mucins increased with growing linear velocity of the surrounding liquid. A. salmonicida bound with higher avidity to skin mucins at a fluid velocity of 2 cm/s (p≤0.05; n = 7) and to distal intestinal mucins at 1.5 cm/s and 2 cm/s fluid velocity (p≤0.05 and p≤0.01, respectively; n = 7). C. A. hydrophila binding to skin mucins was reduced at 2 cm/s fluid velocity (p≤0.05; n = 7). D. A. hydrophila binding was higher at 1.5 cm/s and 2 cm/s fluid velocity compared to the static environment (p≤0.05 and p≤0.01, respectively; n = 5). Data points represent mean±SEM of biological replicates. The results were reproduced twice. Statistics: One-Way ANOVA with Dunnet´s post-hoc test (compared to 0 cm/s velocity).</p