Standard curves for calculation of adherent bacteria from luminescent signals.

Abstract

Luminescence produced by A. hydrophila (A), V. harveyi (B), M. viscosa (C) and Y. ruckeri (D) using the Bac Titer-Glo™ reagent. The standard curves allow for transformation of Signal/noise ratios of luminescent signals into number of adhered bacteria/cm2 surface of the plate well using linear regression. The data points are expressed as mean±SEM and are technical replicates (due to low variation between replicates, the error bars are small, and what looks like symbols in the graph are the error bars). CFU = colony forming unit.</p