MOESM1 of MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection

Abstract

Additional file 1. Flow chart of the miRNA prediction and differentially expressed (DE) miRNA analysis from goat PBMC infected with PPRV at 1.0 multiplicity of infection (MOI). The 49nt sequence tags from Hiseq sequencing will go through the data cleaning analysis first, then the standard analysis will annotate the clean tags into different categories and take those which cannot be annotated to any category to predict the novel miRNA and seed edit of potential known miRNA. After getting miRNA result, target prediction for miRNA and GO enrichment and KEGG pathway for target genes will be analyzed