Specificity of fluorophore - streptavidin detection of biotinylated total human DNA probe in metaphase chromosomes and photostability

Abstract

() Control (no fluorophore – streptavidin conjugate); () streptavidin – Qdot 605 detection of chromosome 1q12 region (vertical and horizontal arrows); () Texas Red – streptavidin detection of biotinylated DNA hybridized to 1q12 (vertical arrows) and () FITC – streptavidin detection of 1q12 sites (vertical arrows). Bar in panel () is 10 μm. () Signal decay upon continuous illumination with fluorescence microscope/mercury illumination in metaphase chromosome band 1q12 during 2 h continuous illumination. Red is Qdot 605, green is Texas Red, and blue is FITC. () Total intensity of whole interphase nuclei during 120 ms illumination (blue bars) and background (red bars). = 3 cells in each. Copyright © 2004 Oxford University Press. Xiao Y, Barker PE. 2004. Semiconductor nanocrystal probes for human metaphase chromosomes. , 32:e28. Reproduced with permission.<p><b>Copyright information:</b></p><p>Taken from "Potential clinical applications of quantum dots"</p><p></p><p>International Journal of Nanomedicine 2008;3(2):151-167.</p><p>Published online Jan 2008</p><p>PMCID:PMC2527673.</p><p>© 2008 Dove Medical Press Limited. All rights reserved</p