TGF-β1 induced cell morphologic changes and migration in KYSE-510 cells.

Abstract

<p>Cells were treated with 5 ng/ml TGF-β1 for indicated time. <b>A</b>: After treatment of TGF-β1 or control, morphologic phenotypes and KYSE-510 cells were examined under an inverted phase-contrast microscope. <i>Scale bars</i>, 20 µm. <b>B</b>: Transwell assay showing TGF-β1-induced cell migration in KYSE-510 cells. Cells were stained with crystal violet (<i>top</i>). The bar graph shows the relative number of migrated cells from three independent experiments (mean±SE, <i>bottom</i>). *, <i>p</i><0.05. <b>C</b>: Measurement of cell viability and proliferation KYSE-510 after TGF-β1 treatment by MTT assay. <b>D</b>: Western blot for N-cadherin and E-cadherin using whole cell extract at 48 hours after TGF-β1 treatment in KYSE510 cells. β-actin served as the loading control. <b>E</b>: RT-qPCR analysis showing the mRNA expression levels of some EMT markers in KYSE-510 cells with or without TGF-β1 treatment for indicated time. Data represent the mean ± SD of triplicate experiments.</p