miR-9 and miR-103 restrain proliferation and affect differentiation marker expression.

Abstract

<p>(<b>A</b>) BrdU incorporation assay in SK-N-BE (grey bars) and SH-SY5Y (black bars) cells transfected with plasmids expressing miR-9, miR-103, a combination of the two, or control (CTRL). Values represent means ± SD of three independent transfections. (<b>B</b>) Immunoblotting of Vgf and N-Myc in SK-N-BE (upper panel) and SH-SY5Y (lower panel) cells transfected with vectors expressing miR-9, miR-103, the two together or control (CTRL). GAPDH was used as loading control. The histograms display the relative quantities (means ± SD) of Vgf and N-Myc versus control cells, from at least three different experiments. *: p-value<0.05; **: p-value<0.01, ***: p-value<0.001.</p