Spatial accumulation of rFaeG_ntd/dsc in transplastomic tobacco plants.

Abstract

<p>(a) Schematic showing the 10 leaves sampled to assess the spatial accumulation of rFaeG_ntd/dsc in transplastomic tobacco plants. (b) Samples examined on SDS-PAGE stained gel (upper panel) and western blot (lower panel). Each lane was loaded with an extract from either ∼2.3 mg of leaf tissue (stained gel), or ∼0.5 mg (immunoblotted gel). WT =  leaf 4 from an untransformed plant. A band of the predicted size (29 kDa, indicated with a black rhomb) corresponding to rFaeG_ntd/dsc was observed in all transplastomic leaf samples, but was absent in the WT. This band was immunoreactive with anti-FaeG serum on the Western blot. kDa - protein molecular weight marker.</p