Southern blot and northern blot analysis of shrimp Ago1 isoforms.

Abstract

<p>(<b>A</b>) Southern blot of shrimp genomic DNA with DIG-labeled Ago1-probe that could detect three Ago1 isoforms or Ago1-fragment 2-probe that was unique to Ago1A and Ago1B. (<b>B</b>) Northern blot of total RNAs extracted from shrimp gills. The probes used were shown on the top. The upper band likely consisted of co-migrated Ago1A and Ago1B transcripts, while the lower band potentially represented the Ago1C transcript.</p