The silencing of <i>Ts-pmy</i> mRNA mediated by siRNA.

Abstract

<p>The adult and larval <i>T. spiralis</i> were transfected with FITC-labeled control siRNA by electroporation (A). Uptake of FITC-labeled siRNA into adult worms (a) and larvae (b) 18 hours after electroporation under a fluorescent microscope. No fluorescence was observed in the untreated group (c). The relative levels of <i>Ts-pmy</i> mRNA in parasites (larvae and adults) 6 days after being electroporated with different siRNAs, as determined with qRT-PCR (B). All of the assays were performed in triplicate, and the data are presented as the mean ± SE. *<i>p</i><0.05 compared with control siRNA.</p