DCs injected at the time of infection with <i>L. mexicana</i> lead to a more robust Th1 response.

Abstract

<p>C57BL/6 mice were infected as before in the ear with <i>L. mexicana</i>, however, one group also received DCs that were derived in vitro. At two weeks post-infection ears and dLNs were processed. (<b>A</b>) Levels of IFN-γ (ng/mL) from the supernatants of single cell suspensions from the dLN of each group that were stimulated for 72 hours with <i>L. mexicana</i> freeze-thaw antigen. (<b>B</b>) Percentage and absolute number of iNOS-producing cells in the ear of <i>L. mexicana</i> infected mice or <i>L. mexicana</i> infected mice that received DCs. Cells are previously gated on live, singlets that are CD11b^hi CD11c⁺. (<b>C</b>) The cellularity of the dLN in <i>L. mexicana</i> infected mice or <i>L. mexicana</i> infected mice that received DCs. The results expressed are the mean percentage (± SD for FACS plots) or the mean number of cells (± SE for bar graphs) of 2–4 mice per group. The results are representative of two experiments. * significantly higher (p<0.05) compared to <i>L. mexicana</i> infected mice.</p