pCMV-UbsHAPQ protects against lethal ASFV challenge.

Abstract

<p>(A) PBMCs from pigs immunized twice (2x) or 4 times (4x) with pCMV-UbsHAPQ or with pCMV, were <i>in vitro</i> stimulated with media (negative control), with the E75 ASFV isolate (10⁵ HAU₅₀/ml), with a mix of the p54, p30 and HA ASFV proteins (6 μg/ml of each one) or with each one of these proteins individually. Values shown for each immunization group correspond to the average number of specific IFNγ-secretory cells detected per million PBMCs. Standard deviations found within each group are also represented. (B) Immunized pigs were challenged with a lethal dose of ASFV (10⁴ UHA of the E75 isolate) and survival record were plotted. Groups immunized with pCMV-UbsHAPQ showed statistically significant differences (*) when compared with the control group (p = 0.0068).</p