TIEG1 associates with the Runx2 promoter in a DNA binding dependent manner.

Abstract

<p>(<b>A–D</b>) Transient chromatin immunoprecipitation (ChIP) assays were performed in U2OS cells transfected with indicated Flag-tagged expression vectors and promoter constructs for 24 hours. Chromatin was prepared, immunoprecipitated with a Flag specific antibody and amplified by both real-time PCR (<b>A and C</b>) and semi-quantitative PCR (<b>B and D</b>). Real-time PCR analysis was utilized for quantitation purposes and the data are expressed as the abundance of the Runx2 promoter relative to cells transfected with a control expression construct. All data were normalized using input samples. <i>Asterisks</i> denote significance at the p<0.05 level (ANOVA) compared with controls. δ denotes significance at the p<0.05 level (ANOVA) between indicated promoter constructs. The products obtained by semi-quantitative PCR were separated using agarose gel electrophoresis.</p