TolC is required for extreme-acid survival in a strain defective for eight MDR efflux inner membrane pumps.

Abstract

<p>Strains N7829 (K-12 derivative), M5567 (N7829 <i>tolC</i>::<i>kan</i>), M6293 (N7829 <i>acrB</i>::<i>frt acrD</i>::<i>frt emrB</i>::<i>frt emrY</i>::<i>frt macB</i>::<i>frt mdtC</i>::<i>frt mdtF</i>::<i>frt acrEF</i>::<i>spc</i>), and JLS1042 (M6293 <i>tolC</i>::<i>kan</i>) were grown overnight to stationary phase in LBK buffered at pH 5.5 (100 mM MES), diluted into LBK pH 2, and exposed for 2 hours at 37°C. Grey bars represent the parent strain listed with the additional kanamycin resistance insertion in <i>tolC</i>. Error bars = SEM, n = 6.</p