Antigen presentation efficiency is normal in I-A-β-K225R-EGFP BMDCs.

Abstract

<p><i>A</i>, BMDCs were incubated with the indicated concentrations of ovalbumin (Ova) for one hour and subsequently fixed. T cells from OTII mice were incubated with the fixed BMDCs overnight and upregulation of CD69 on T cells was assessed by flow cytometry. <i>B</i>, Assays were performed as in <i>A</i>. BMDCs were incubated with 2 µg/ml Ova for the indicated times prior to fixation. These experiments were performed twice with a total of 4 mice of each genotype; representative data are shown.</p