HPV interacts with syndecan-1 on human sperm.

Abstract

<p><b>a</b>. Cytofluorimetric analysis with mouse anti-human CD138 (syndecan-1), FITC conjugated on sperm before and after treatment with Heparinase-III. Samples without anti CD138 were used as negative controls. <b>b</b>. Immunofluorescence analysis (confocal fluorescence microscope) for syndecan-1 (CD138, FITC conjugated of sperm before and after treatment with Heparinase-III. Upper panel: negative control (omission of CD138); central panel: syndecan-1; lower panel: syndecan-1 after Heparinase-III treatment. <b>c</b>. Cytofluorimetric analysis of sperm exposed to HPV16 L1 capsid protein, treated or not with Heparinase-III and analyzed with mouse monoclonal HPV16 L1 antibody. Samples incubated with secondary phycoerythrin anti-mouse antibody alone were used as negative controls. <b>d</b>. Immunofluorescence analysis for HPV L1 capsid protein (confocal fluorescence microscope) in sperm exposed to HPV16 L1 treated or not with Heparinase-III and analyzed with mouse monoclonal antibody anti HPV16 L1 and DAPI. <b>e</b>. Colocalization analysis of HPV L1 capsid protein and syndecan-1 by immunofluorescence (confocal fluorescence microscope) of sperm exposed to HPV16 L1 treated or not with Heparinase-III and analyzed with mouse anti-human CD138 (syndecan-1) monoclonal antibody, FITC conjugated and mouse monoclonal antibody anti HPV16 L1 and DAPI.</p