<i>In vitro</i> selection of maC46-expressing primary human T cells.

Abstract

<p>Human CD4⁺ T cells were transduced with either a control lentiviral vector expressing GFP alone (M420) or a vector expressing maC46 and GFP. On day 5 after transduction, cells were diluted with non-transduced cells to achieve 10% GFP⁺ cells, and the mixtures were infected with HIV-1_D117/II. Data are presented as the mean ± the standard error of the mean (SEM) of triplicate samples. A. Flow cytometric analysis of the percentage of CD4⁺ T cells as a percentage of all viable cells. Infected cultures (closed symbols) showed a sustained loss of CD4⁺ T cells. B. Preferential survival of maC46-expressing CD4⁺ T cells following HIV-1 infection. C. Increased expression of GFP on PM-1 cells transduced with the maC46-GFP vector (M589) after HIV-1-infection. The geometric mean of GFP expression (Geo mean) on human CD4⁺ T cells was analyzed at the indicated time points.</p