Dysfunction in Type-I IFN Production or Signaling in Normal and Tumor Cell Lines

Abstract

<div><p>(A) Production of IFN-β. Cell lines (NHBEs, HCT 116, C33A, and A2780) were infected with MV or <i>WR-delB18R</i> at an MOI of 5.0, or treated with LPS at 5 μg/ml; medium was collected after 18 h, and IFN-β levels determined by ELISA.</p> <p>(B) Response to IFN-β. Cell lines (NHBEs, HCT 116, and A2780) were treated with IFN-β (blue graphs) or PBS (red graphs), and fixed and permeabilized 15 min later. Levels of phospho-STAT1 were determined by flow cytometry.</p></div