RNA Synthesis Phenotype of the Alb <i>ts</i>22 Mutant

Abstract

<p>RNA synthesis was determined (A) using 1 h pulse labels with ³H-uridine in the presence of dactinomycin, given to MHV-A59-, Alb <i>ts</i>22-, and Alb 22R-infected cells 1–6 hpi at 40 °C or 5–14 hpi at 30 °C; █, 40 °C, wt MHV-A59; ▴, 40 °C, Alb 22R; •, 40 °C, Alb <i>ts</i>22; □, 30 °C, wt MHV-A59; ▵, 30 °C, Alb 22R; ○, 30 °C, Alb <i>ts</i>22, or (B) using 30 min pulse labels with ³H-uridine in the presence of dactinomycin, given to MHV-A59-, Alb <i>ts</i>22-, and Alb 22R-infected cells after shift from the permissive to the non-permissive temperature at 13 hpi; █, wt MHV-A59; ▴, Alb 22R; •, Alb <i>ts</i>22.</p