Effect of ERβ downregulation on the cell growth rate.

Abstract

<p>(A) Western blot results after transfection of siERβ (siERβ1, siERβ2, siERβ3, or siERβ123) or scramble control (nonsense siRNA sequence that would not affect ERβ expression). Cells transfected with siERβ showed ERβ protein downregulation. (B) mRNA expression reduced after 786-O transfection with different concentrations of siERβ. (C) Cell growth of cells transfected with scramble control or siERβ after estrogen stimulation (10 nM) was observed by MTT assay.</p