Effects of RAR agonists on <i>Csn3</i> expression in P19 cells.

Abstract

<p>(<b>A</b>) RT-PCR analysis of the expression of <i>Csn3</i> and genes encoding the RAR subtypes (<i>Rara</i>, <i>Rarb</i>, and <i>Rarg</i>) during the neural differentiation in P19 cells. Total RNA was isolated from P19 cells at the time indicated following ATRA treatment, and used for cDNA synthesis. PCR analysis was performed with primer sets specific for <i>Csn3</i>, each <i>Rar</i> or for <i>Gapdh</i>. PCR products were then subjected to electrophoresis through a 1.5% agarose gel and subsequently stained with ethidium bromide. This experiment was repeated three times with similar results, and representative pictures are shown here. (<b>B</b>) RT-PCR analysis of <i>Csn3</i>, <i>Rara</i>, <i>Rarb</i>, and <i>Rarg</i> mRNA expression in P19 cells treated with RAR agonists. Total RNA was extracted from P19 cells treated with 100 nM Am80 (RARα agonist; upper panel) or 100 nM AC-41848 (RARγ agonist; lower panel), and <i>Csn3</i> and genes encoding the RAR subtypes expressions were evaluated by RT-PCR analysis. <i>Gapdh</i> was used as a loading control. Numbers in parentheses next to the gene symbols indicate the number of PCR cycles. RT-PCR experiments were repeated at least three times with similar results.</p