Binding of RARα to the <i>Csn3</i> promoter region containing the DR5 RARE motif.

Abstract

<p>(<b>A</b>) Schematic illustration of the mouse <i>Csn3</i> promoter region. The <i>Csn3</i> DR5 RARE (filled box) and exon 1 (open box) are shown. Arrows under the nucleotide indicate the positions of the PCR primers used for chromatin immunoprecipitation (ChIP) assays. The numbers indicate position relative to the transcriptional start site (+1). (<b>B</b>) ChIP assays of RARα in <i>Csn3</i> promoter region in P19 cells treated with ATRA. DNA sequences within chromatin that had been immunoprecipitated with anti-RARα antibody (lane 2) or non-specific mouse IgG (negative control, lane 3) were amplified using primer pairs for the region including the DR5 RARE in <i>Csn3</i> promoter (−206 to +1) or the distal region from the target site (−739 to −575). Aliquots of the chromatin before immunoprecipitation were used as a positive control (Input, lane 1). Lane 4 contains non-template DNA.</p