Pathogenicity test for <i>xop</i> mutants of <i>Xoo</i> KXO85 in rice.

Abstract

<p><b>A</b>. Disease severity of each <i>xop</i> mutant in 3-month-old rice leaves. W, water; 85, wild-type KXO85; Q, KXO85 <i>xopQ</i>_<i>KXO85</i>::EZ-Tn<i>5</i>; X, KXO85 <i>xopX</i>_<i>KXO85</i>::EZ-Tn<i>5</i>; P1, KXO85 <i>xopP1</i>_<i>KXO85</i>::EZ-Tn<i>5</i>; P2, KXO85 <i>xopP2</i>_<i>KXO85</i>::EZ-Tn<i>5</i>; N, KXO85 <i>xopN</i>_<i>KXO85</i>::EZ-Tn<i>5</i>. <b>B</b>. Disease severity of the <i>xopN</i>_<i>KXO85</i> mutants in the flag leaves of rice grown in a paddy field. W, water; 85, KXO85; N, KXO85 <i>xopN</i>_<i>KXO85</i>::EZ-Tn<i>5</i>; and N^C, KXO85 <i>xopN</i>_<i>KXO85</i>::EZ-Tn<i>5</i> (pML122G2). Photographs were taken and lesion lengths were determined 21 days after inoculation. Vertical error bars indicate the standard deviations (SD). The data are the averages of 12–15 replicates for each treatment. Columns and lines not connected by the same letter are significantly different (P<0.05) as determined by a one-way ANOVA (P<0.001) followed by post hoc Tukey HSD analysis. <b>C</b>. Bacterial growth patterns of the KXO85, <i>xopN</i>_<i>KXO85</i> mutant, and complemented <i>xopN</i>_<i>KXO85</i> mutant strains in flag leaves of wild-type Dongjin. The data are shown as the average values for three replicates; vertical bars indicate the error ranges (±SD). The bacterial populations were assessed every 3 days after inoculation. Different letters at day 21 indicate significant differences (P<0.05) as determined by a one-way ANOVA (P<0.001) followed by post hoc Tukey HSD analysis.</p