Effects of CysLT₁R antagonists on HCT-116 cell proliferation and cell cycle.

Abstract

<p>Cell proliferation was measured using the WST-1 cell proliferation assay, and absorption of the samples was measured at 440 nm. Cells were treated with (<b>A</b>) ZM198,615 or (<b>B</b>) Montelukast for 24, 48, 72, or 96 h. (<b>C</b>) Cell cycle analysis was carried out with propidium iodide staining. Percentages of the total cell population in different phases of the cell cycle were analyzed by flow cytometry. The quantitative data shown are the mean ± SEM from three separate experiments. <i>*P</i><0.05, <i>**P</i><0.01, <i>***P</i><0.001 by paired <i>t</i> test.</p