1H-MRS and GC-MS metabolic profiles of bone marrow and peripheral blood samples at the time of ALL diagnosis.

Abstract

<p>Representative spectra of BM (blue line) and PB (red line) specimens. Spectra were acquired from (A) 1H-MRS analysis of filtered polar fractions, (B) 1H-MRS analysis of recovered whole lipid fractions, and (C) GC-MS analysis of FFA extracts. Metabolites with the greatest difference between BM and PB are labeled and include alanine (Ala), free cholesterol (CHOL), cholesterol esters (CHOLest), choline (Cho), formate (For), glucose (Glc), glutamate (Glu), glutamine (Gln), lactate (Lac), histidine (His), hypoxanthine (Hpx), palmitic acid, oleic acid, triacylglyceride (TAG), and uridine (Ur). Other abbreviations used are: (2HB), 2-hydroxybutyrate; (3HB), 3-hydroxybutyrate; (2Og), 2oxo-glutarate; (2Oic), 2oxo-isocaproate; (BAA), branched amino acids; (Car), carnitine; (Cho), choline; (CHOL), free cholesterol, (CHOLest), cholesterol esters; (For), formate; (Fum), fumarate; (Glyc), glycerol; (GPCho), glycero-3-phosphocholine; (Hpx), hypoxanthine; (Lac), lactate; (Niac), niacinamide; (Pglu), pyroglutamate; (Pyr), pyruvate; (Ur), uridine; (Pdx), pyridoxine; (TAG), triacylglyceride; (T-Chol), total cholesterol.</p