EUE active components geniposide and aucubin enhance lysosomal enzyme activation.

Abstract

<p>Cells were treated with 500 µM palmitate in the presence or absence of 10 µg/mL aucubin or geniposide for 24 hours. Lysosomal V-ATPase activity was measured as described in Materials and Methods (A). Cells were treated with 500 µM palmitate in the presence or absence of 10 µg/mL aucubin or geniposide for 24 hours followed by exposure to 5 µM LysoTracker and image acquisition. The fluorescence was quantified (B). Acridine orange solution and valinomycin were added to cell monolayers and intravesicular H⁺ uptake was initiated by the addition of Mg-ATP (C); the fluorescence was quantified at 24 hours (C; right). Cells were treated with 500 µM palmitate in the presence or absence of 10 µg/mL aucubin or geniposide for 0, 12, 24, or 48 hours and the level of α-galactosidase, α-mannosidase, or acid phosphatase was measured (D). ^*<i>p</i><0.05, significantly different from palmitate-treated condition. Con; control, Pal.; palmitate.</p