Increased cellular infiltration into the lesion of LCMV immune <i>L. major</i> infected mice.

Abstract

<p>B6 mice were infected with LCMV Armstrong or left uninfected. After 30 days, mice were infected with <i>L. major</i>. After 4 weeks, infected ears were taken and fixed for histological analysis (A–D) or digested for flow cytometric analysis (E–H). Fixed ear tissue was sectioned and stained with hematoxylin and eosin (H&E) and imaged at 10× magnification or 40× magnification (inlay)(A). A Verhoeff's stain was also done on fixed ear tissue from LCMV immune <i>L. major</i> infected mice to stain for collagen and imaged at 10× magnification (B–D). Scale bars represent 100 µm. Damage unique to the LCMV immune lesions is highlighted by arrows, specifically epidermal thickening (B), cartilage destruction (C), epidermal ulceration (C), and loss of dermal collagen (D). Digested ears were stained for surface markers of monocytes and neutrophils (E), T cells (G), or NK cells (I). Results are shown in the form of representative plots (E, G, and I) and total cell numbers (F, H, and J). Samples were pregated on live, CD45+, TCRβ−, CD11b+ events (E) or on live, CD45+ events (G and I). Histological data are representative of two independent experiments (n = 4–5 mice per group; A–D). Flow cytometric data is a representative of five independent experiments (n = 4–5 per group; E–J). Percentages are shown as mean ± SEM.</p