Nuclear export of L-periaxin is inhibited by LMB.

Abstract

<p>(A) RSC96 cells were transfected with the plasmids encoding EGFP-tagged L-PRX and PDZ-cyclin A1. The cells in the second and fourth panels were treated with 37 nmol/L LMB for 1 h after 7 h post-transfection. EGFP-L-PRX and EGFP-PDZ-cyclin A1 localization was observed by Delta Vision. Scale bar = 25 μm. (B) The cytosol and nucleus were separated and determined the distribution of protein in the two fractions by Western blotting. β-actin and Histone served as an internal control for cytosol and nucleus, respectively. (C) Quantitative analysis of the distribution of EGFP fusion proteins in the two fractions by normalized to the internal control level. *P<0.05.</p