Osteogenesis of rabbit annulus fibrosus cells <i>in vitro</i>.

Abstract

<p>Rabbit annulus fibrosus cells were cultured in monolayer, for four weeks, with osteogenic medium or growth medium. A) Alizanin Red staining shows mineralization; the signal was quantified using optical density at 570 nm. B) Expression of Runx-2, BMP-2, and Osteopointin genes markedly increased in cells cultured with osteogenic medium compared to the growth medium. *, p<0.05</p