PRL1 binds pri-miRNAs <i>in vitro</i> and <i>in vivo</i>.

Abstract

<p>(A) Proteins used for <i>in vitro</i> RNA binding assay. Purified MBP and MBP-PRL1 proteins were resolved on SDS-page gel and stained by Coomassie Blue. The lower bands in MBP-PRL1 line are degraded MBP-PRL1 since they can be detected by anti-MBP antibody. M: marker. (B) PRL1 binds <i>MIR162b</i>, <i>pre-miR162b</i> and ssRNA <i>in vitro</i>. <i>MIR162b</i>, <i>pre-miR162b</i> and ssRNA were produced by <i>in vitro</i> transcription. SsRNA: single stranded-RNA; DsRNA: double stranded-RNA. (C) PRL1 binds pri-miRNAs <i>in vivo</i>. NoAb: No antibody control. Transgenic plants harboring <i>pPRL1::PRL1-YFP</i>, <i>pTGH:</i> TGH-YFP or <i>YFP</i> transgene were used for RIP assay with anti-YFP anti-bodies. Ten percent immunoprecipitates and two percent input proteins were analyzed by western blot. Five percent RNAs were used as input RNA.</p