PRL1 positively influences pri-miRNA levels.

Abstract

<p>(A) The abundance of pri-miRNAs in inflorescences of <i>prl1-2</i> and Col. The transcript levels of pri-miRNAs in <i>prl1-2</i> were determined by quantitative RT-PCR (qRT-PCR), normalized to that of <i>UBQUITIN5</i> (<i>UBQ5</i>) and compared with those in Col. Value of Col was set to 1. Error bars indicate standard deviation of three technical replications. **: P<0.01. (B) GUS expression in <i>PRL1⁺</i> and <i>prl1-2</i> harboring <i>pMIR167a::GUS</i>. <i>PRL1⁺</i>: <i>PRL1/PRL1</i> or <i>PRL1/prl1-2</i>. Twenty plants containing GUS were stained for each genotype and an image was shown. (C) Relative <i>GUS</i> mRNA levels in <i>PRL1⁺</i> and <i>prl1-2</i> harboring <i>pMIR167a</i>::GUS. <i>GUS</i> transcript levels were determined by qRT-PCR and normalized to <i>UBQ5</i>. Value of <i>PRL1⁺</i> was set to 1. Standard deviation of three technical replications was shown as error bars. P = 0.11 (t-test).</p