The <i>estA2</i> promoter is repressed by H-NS.

Abstract

<p>A) The panel shows different P<i>estA2::lacZ</i> fusions. The <i>lacZ</i> gene is shown as a red arrow and the <i>estA2</i> gene is shown as a blue arrow. P<i>estA2</i> is illustrated using a bent arrow and the CRP binding site is shown as an orange box. B) H-NS binds to P<i>estA2</i> only in the presence of flanking DNA. ChIP-PCR was used to measure binding of H-NS to the different P<i>estA2</i> derivatives cloned in pRW50. PCR products were generated using primers that could detect P<i>estA2</i> in the context of both the 93 bp fragment and the longer 460 bp fragment. C) The values are β-galactsidase activity values for lysates of M182, or M182Δ<i>hns</i>, carrying the different P<i>estA2</i> derivatives. Assays were done in LB medium.</p