Antisense probes for the experimental control of stereospecificity.

Abstract

<p>l-ribozyme solution (2 μM) was artificially contaminated with 0.01% enantiomeric d-ribozyme (0.2 nM) and cleavage of d-RNA substrate was analyzed after 5 h at 37°C. d- and l-antisense probes (2 nM) were added to control stereospecificity. Assays were performed in 50 mM Tris, pH 7.5, 10 mM MgCl₂. RNA substrate and its 5′-hydrolysis products were visualized via a 5′-fluorescein tag. Ribozymes and DNAzymes were stained with ethidium bromide. Data is representative of two independent experiments.</p