Effects of migration and culture on gene and surface protein expression by neutrophils.

Abstract

<p>A. Total mRNA extracted from neutrophils (pg/cell). Freshly isolated neutrophils (Fresh) were compared to neutrophils recovered from a gel 1h, 3h or 24h after migrating through EC treated with 100U/ml TNF. B,C. mRNA and surface protein expression of β1-integrin. D,E. mRNA and surface protein expression of β2-integrin. F,G. mRNA and surface protein expression of β3-integrin. H,I. mRNA and surface protein expression of ICAM-1. In B-I, values for neutrophils recovered from a gel at 1 h, 3 h or 24 h, are expressed relative to values for freshly isolated cells. Data are mean ± SEM from 3 to 10 independent experiments. * = p<0.05, ** = p<0.01 compared to freshly-isolated cells by paired t test.</p