Effects of migration and culture on phagocytosis by neutrophils.

Abstract

<p>Neutrophil phagocytosis was analysed by measuring intensity of fluorescence of cells incubated with pHrodo <i>E. coli</i> BioParticles. Freshly isolated neutrophils (without or with collagenase treatment) were compared to neutrophils recovered from a gel 1h or 24 h after migrating through EC treated with 100U/ml TNF. A. Samples were incubated with bioparticles for 10, 30 or 60min before being fixed for flow cytometry. B. Confocal microscopic images of neutrophils recovered after 1h and incubated with bioparticles for 60min, imaged in the same plane for (i) bisbenzamide-stained nuclei or (ii) fluorescent intracellular bioparticles. Data are mean ± SEM from 3 experiments. ** = p<0.01compared to freshly-isolated cells by paired t test.</p