Coverage profiles along <i>A</i>. <i>thaliana</i> chromosomes and TAIR10 annotated CDS.

Abstract

<p>(A) Distribution of RNA-seq read density along chromosome length is shown for total RNA-seq (left) and polyribosomal RNA-seq (right). The y axis represents the log2 scale of median read density. (B) Distribution of the RNA-seq read coverage along the length of the transcriptional unit. The log2 scale of median depth of coverage along the length of each individual TAIR10 annotated cDNA was calculated and plotted against the relative length of the transcriptional unit for the total RNA-seq and polyribosomal RNA-seq. (C) Coverage over the length of TAIR10 annotated CDS. Box-and-whisker plots depict the coverage calculated as the percentage of bases along the length of the cDNA sequence that was supported by reads from the total and polyribosomal RNA-seq datasets. The bottom and top of the boxes represent the 25^th and 75^th quartiles, respectively. The lines within boxes represent the medians.</p