Levels of constructed protein-induced IgG, IgG1, and IgG2c antibodies in the sera of <i>B6;129S-Ldlr</i>^{<i>tm1Her</i>}<i>Apob</i>^{<i>tm2Sgy</i>}<i>/J</i> mice at 2 weeks and 12 weeks respectively, after the first immunization and in controls (GST-Den-immunized mice).

Abstract

<p>The mean optical densities (ODs) and SEM obtained from plasma samples of constructs AHHC, RHHC, and RPHC-immunized mice on Cpn peptide (C)-, hHSP60^153-163 (H)-, hHSP60^303-312 (H)-, C5aR-peptide (R)-, ApoB peptide (A)-, PAR-1(P)-coated ELISA plates are shown. (A) IgG at the dilution ratio: 1:100. (B) IgG1 at the dilution ratio: 1:6250. (C) IgG2c at the dilution ratio: 1:50. (D) Cross reaction between human ApoB peptide-induced antiserum and Cpn peptide. (E) Cross reaction between human ApoB peptide- and hHSP60^303-312 peptide-induced antiserum and their antigens. (F) Cross reaction between ApoB peptide-induced antiserum and antigens of either PAR-1 peptide or C5aR peptide. (G) Cross reaction between C5aR peptide-induced antiserum and antigens of either ApoB peptide or PAR-1 peptide. Antiserum used for cross-reaction was taken at week 8.</p