Evidence of an eosinophil trafficking defect in HFD mice.

Abstract

<p>(A) Ileal sections from normal chow or 7 day HFD mice were stained for in situ apoptotic cells using TdT-Fluor staining protocol. Minimal apoptosis was detected in either condition relative to nuclease treated positive control. (B) Bone marrow and (C) peripheral blood leukocytes were isolated from 5 week HFD mice and proportions of eosinophils determined by flow cytometry. D) Expression of CCL11 and CCL24 genes in the small intestinal mucosa of 1 week HFD or normal chow mice. In all graphs each data point represents an individual mouse from one experiment. *p<0.05, **p<0.01, ***p<0.001 Mann-Whitney U test.</p