TH, DBH and PNMT protein expression in tumors.

Abstract

<p>(A) Western blot of sample tissues from NorAd PHEO (A, n = 14), mixed PHEO (B, n = 21) and PGL (n = 12). Volumes loaded were 10μl of a 20% w/v extract. E and NE values corresponding to the samples analysed by western blot are given in nanomoles/gram of tissue, lower quantification limit was set at 1nmole/gram. (B) Quantification of the signal obtained by western blot. Results are given as QL (corresponding band pixel values)-BG (background pixel value), and calculated values are divided by the β-actin signal value and reported on the y-axis. Geographic mean and standard deviation for each group are reported in supporting information (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125426#pone.0125426.s006" target="_blank">S6 Table</a>). ***<i>P</i><0.001, **<i>P</i><0.01, *<i>P</i><0.05, ns; not statistically significant.</p