<p>The interaction of <i>Lactobacillus acidophilus</i> and time on MLCP protein concentrations was not significant. (A) ELISA analysis showed that the concentrations of MLCP in intestinal smooth muscle were significantly increased after TBI, **<i>P</i> < 0.01 compared with control. <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated increase of MLCP, #<i>P</i> < 0.05 compared with TBI. (B) Immunohistochemistry analysis showed that marked increases in MLCP were observed in intestinal smooth muscle after TBI, and treatment with <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated increases of MLCP.</p