The fetal kidney cells inhibit oxidative stress and apoptosis in kidneys with ischemic injury.

Abstract

<p>(A, B) mRNA expression levels of GR and GPx. (C) Representative immunoblots showing the expression of anti-oxidative biomarkers viz. HO-1 and NQO-1. (D, E) Bar diagrams showing semi quantitative densitometry of expression of the HO-1 and NQO-1. (F) Representative immunoblots showing the expression of apoptosis related biomarkers viz. Bax, Bcl2, caspase 3 and cytochrome c in kidney tissues of sham operated, saline treated and fetal kidney cells treated groups. (G-I) Bar diagrams showing semi quantitative densitometry of a ratio of the expression of Bax/Bcl2 and expression of the caspase 3 and cytochrome c. Comparative gene expression ratio calculated by referring each gene to β-actin as an internal control. Densitometric analysis applied for comparison of relative protein expression and represented in densitometric arbitrary units (a. u.). Values expressed Mean±SEM. *p<0.05 vs. sham operated group. #p<0.05 vs. saline treated group.</p