TSCs from T1r3 KO mice can also elicit synergistic responses.

Abstract

<p>Ca²⁺ responses of T1r3 KO TSCs to the four L-amino acid sets are shown. Each L-amino acid set consisted of one of the four L-amino acids (MPG (10mM), Ser (20mM), Arg (10mM), or Gln (10mM), respectively), IMP (1mM), and the MIX of L-amino acid+IMP. [A, D, G, J] Representative TSC responses when the magnitudes of MIX responses were greater than the summation of individual L-amino acid and IMP responses, i.e., the MIX responses were synergistic. [B, E, H, I] Representative TSC responses where the magnitudes of MIX responses were greater than or equal to the response of the L-amino acid or IMP individually, but not greater than the summation of the individual L-amino acid and IMP responses, i.e., the MIX responses were not synergistic. [C, F, I, L] Mean±SEM response for the L-amino acid sets exhibiting synergistic and non-synergistic responses. We were unable to conclude anything about Gln set as only 2 cells responded synergistically to Gln+IMP. Numbers in parenthesis are the number of cells. Mann Whitney test was used for statistical testing. ***P<0.001, **P<0.01, *P<0.05.</p