The CTP-TMR and HMGA2Δ95–108 co-eluting in gel-filtration chromatography.

Abstract

<p>The CTP-TMR was prepared as described under “Materials and Methods” and incubated with HMGA2Δ95–108 at 24°C for 30 min in BPES buffer. The CTP-TMR and HMGA2Δ95–108 mixture was then subjected to a Sephacryl S-100 HR filtration column (1×50 cm) equilibrated with BPES buffer. Gel filtration profile of the CTP-TMR binding to HMGA2Δ95–108 was monitored by a graph of OD₅₅₆ versus elution volume (A) and a 15% SDS PAGE gel (B). Lanes 1 to 8 of the SDS-PAGE gel (B) correspond to the fractions 1 to 8 labeled in panel a. Free HMGA2Δ95–108 and the CTP-TMR were eluted at 22 and 30 ml respectively in the column.</p