(A) Amino acid sequence of mouse HMGA2.

Abstract

<p>The positively charged “AT hooks” and the negatively charged C-terminus are highlighted by underlines and a box, respectively. <b>(B) The experimental (open dots with dotted line) and calculated (solid line) CD spectra of HMGA2 in BPES buffer.</b> The CD spectra were calculated by the program CONTIN as described in the text. The solid dots are residuals. <b>(C) Gel filtration chromatography profile of HMGA2 in solution.</b> Recombinant HMGA2 was resolved by gel filtration in the Sephacryl S-100 column as described under “Materials and Methods.” V₀ is the void volume; A, C, and R represent the elution volume of albumin, chymotrypsinogen A, and ribonuclease A, respectively.</p