Angptl4 was expressed at an earlier stage of podocyte injury in ADR rats, and tacrolimus diminished glomerular and urinary Angptl4 expression.

Abstract

<p><b>(A)</b> Immunofluorescence of glomerular Angptl4 in normal, tacrolimus-treated and untreated ADR rats. Scale bars: 50 μm. <b>(B)</b> Quantifications of the fluorescence staining intensities of glomerular Angptl4 in normal, tacrolimus-treated and untreated ADR rats. <b>(C)</b> Western blot of glomerular Angptl4 expression in ADR rats. <b>(D)</b> Western blot of urine Angptl4 excretion in ADR rats. <b>(E)</b> Quantification of the western blot of glomerular Angptl4 expression in ADR rats. <b>(F)</b> Quantification of the western blot of urine Angptl4 excretion. <b>(G)</b> Urine Angptl4 ELISA for ADR rats. <b>(H)</b> Serum Angptl4 ELISA for ADR rats. <b>(I)</b> Quantification of the real-time PCR of glomerular Angptl4 in ADR rats. <b>(J)</b> Relationship between glomerular Angptl4 and glomerular desmin in ADR rats. <b>(K)</b> Relationship between glomerular Angptl4 and 24-hour urinary protein excretion in ADR rats. <b>(L)</b> Relationship between glomerular Angptl4 and glomerular synaptopodin in ADR rats. Con, normal rats; Untreated, ADR rats without treatment; TAC, ADR rats with tacrolimus treatment. ##P<0.05 compared with normal rats; #P<0.01 compared with normal rats; *P<0.01 compared with untreated ADR rats. The arrow indicates that tacrolimus treatment was initiated on day 14.</p