Evaluation of amplification bias of droplet MDA.

Abstract

<p>Distributions of sequencing coverage of MDA products from single <i>Escherichia coli</i> cells (n = 3) were compared between in-tube MDA (left column) and droplet MDA (right column). Each graph shows the results of independent reactions. The averaged sequencing coverages were calculated from raw sequencing reads that mapped to with <i>E</i>. <i>coli</i> reference genome within 1-kb windows. Sequencing reads were normalized to 60× sequencing effort in each experiment.</p