Deletion of conserved motifs (grey area) from the predicted fish regions results in change of enhancer activity in both tested constructs.

Abstract

<p><b>Schematic on the right shows the motif configuration in the human and medaka locus for hs1344 and hs865, respectively. The full grey area is deleted from the medaka enhancer and the remaining sequence tested for reporter expression. Images on the left show the reporter activity of the medaka constructs prior to and after the deletion.</b> Hs1344 ol2-1delta gains two symmetrical domains in the midbrain (red arrowheads), while hs865 ol2-1delta shows a loss of expression in the central part of the original domain.</p