Meta-analysis work flow.

Abstract

<p>The publically available raw microarray data of Head and Neck Squamous Cell Carcinoma (HNSCC) series were downloaded and grouped and analyzed in Genespring statistical software. Normalization was carried out on the samples, which were then grouped into tumor and normal prior to performing the gene level experiment. Principal Component Analysis (PCA) was performed to remove the discordant samples. Fold change and <i>p</i> value were calculated to obtain the significant gene entities. These statistically significant entities were used for further analysis; database annotations (Gene Ontology, STRING and miRWALK) and patient validation (Experimental validation by Quantitative Real Time PCR (qPCR) and The Cancer Genome Atlas (TCGA)).</p