Kin2’s localization to the polarity site is essential for its multicopy suppression of late secretory mutants.

Abstract

Cells of strains JGY27B (sec1-1), JGY28B (sec2-41), JGY82A (sec15-1) carrying pRS426 (Vec), pRS426-KIN2, pRS426-KIN2-NT, pRS426-KIN2-N7, and pRS426-KIN2-N5 were spotted on SC-Ura plate at 1:10 serial dilution and incubated at permissive (24°C) and their respective restrictive temperatures. Pictures were taken after 4 days.</p